SOX2 (N-term) Peptide
产品名称： SOX2 (N-term) Peptide
英文名称： SOX2 (N-term) Peptide
产品编号： P0843
产品价格： 0
产品产地： 台湾
品牌商标： Abnova
更新时间： null
使用范围： null
亚诺法生技股份有限公司（Abnova)
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- 所在区域 : 台湾
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- Specification
- Product Description:
- Rabbit polyclonal antibody raised against synthetic peptide of SOX2.
- Immunogen:
- A synthetic peptide (conjugated with KLH) corresponding to N-terminus of human SOX2.
- Host:
- Rabbit
- Reactivity:
- Human
- Form:
- Liquid
- Purification:
- Ammonium sulfate precipitation
- Storage Buffer:
- In PBS (0.09% sodium azide)
- Storage Instruction:
- Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.
- Recommend Usage:
- ELISA (1:1000)
Western Blot (1:50-100)
Immunohistochemistry (1:10-50)
Immunofluorescence (1:10-50)
Flow cytometry (1:10-50)
The optimal working dilution should be determined by the end user.
- Note:
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Publication Reference
- 1.
- Crystal structure of a POU/HMG/DNA ternary complex suggests differential assembly of Oct4 and Sox2 on two enhancers.
Remenyi A, Lins K, Nissen LJ, Reinbold R, Scholer HR, Wilmanns M.Genes Dev. 2003 Aug 15;17(16):2048-59.
- 2.
- POU5F1 (OCT3/4) identifies cells with pluripotent potential in human germ cell tumors.
Looijenga LH, Stoop H, de Leeuw HP, de Gouveia Brazao CA, Gillis AJ, van Roozendaal KE, van Zoelen EJ, Weber RF, Wolffenbuttel KP, van Dekken H, Honecker F, Bokemeyer C, Perlman EJ, Schneider DT, Kononen J, Sauter G, Oosterhuis JW.Cancer Res. 2003 May 1;63(9):2244-50.
- 3.
- Formation of pluripotent stem cells in the mammalian embryo depends on the POU transcription factor Oct4.
Nichols J, Zevnik B, Anastassiadis K, Niwa H, Klewe-Nebenius D, Chambers I, Scholer H, Smith A.Cell. 1998 Oct 30;95(3):379-91.
- Applications
- Western Blot (Cell lysate)
- Western blot analysis of SOX2 polyclonal antibody (Cat # PAB3876) in 293 cell line lysates (35 ug/lane). SOX2 (arrow) was detected using the purified polyclonal antibody.
- Western Blot (Transfected lysate)
- Western blot analysis of SOX2 (arrow) using rabbit SOX2 polyclonal antibody (Cat # PAB3876). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the SOX2 gene (Lane 2) (Origene Technologies).
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
- Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with SOX2 polyclonal antibody (Cat # PAB3876) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
- Entrez GeneID:
- 6657
- Protein Accession#:
- NP_003097;P48431
- Gene Name:
- SOX2
- Gene Alias:
- ANOP3,MCOPS3,MGC2413
- Gene Description:
- SRY (sex determining region Y)-box 2
- Gene Ontology:
- Hyperlink
- Gene Summary:
- This intronless gene encodes a member of the SRY-related HMG-box (SOX) family of transcription factors involved in the regulation of embryonic development and in the determination of cell fate. The product of this gene is required for stem-cell maintenance in the central nervous system, and also regulates gene expression in the stomach. Mutations in this gene have been associated with optic nerve hypoplasia and with syndromic microphthalmia, a severe form of structural eye malformation. This gene lies within an intron of another gene called SOX2 overlapping transcript (SOX2OT). [provided by RefSeq
- Other Designations:
- SRY-related HMG-box gene 2,sex-determining region Y-box 2,transcription factor SOX2