LATS1 (Human) Recombinant Protein
产品名称： LATS1 (Human) Recombinant Protein
英文名称： LATS1 (Human) Recombinant Protein
产品编号： P5573
产品价格： 0
产品产地： 台湾
品牌商标： Abnova
更新时间： null
使用范围： null
亚诺法生技股份有限公司（Abnova)
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- Specification
- Product Description:
- Human LATS1 (NP_004681.1, 589 a.a. - 1130 a.a.) partial recombinant protein with GST tag expressed in baculovirus infected Sf21 cells.
- Theoretical MW (kDa):
- 90
- Form:
- Liquid
- Preparation Method:
- Baculovirus infected insect cell (Sf21) expression system
- Purification:
- Glutathione sepharose chromatography
- Purity:
- 66 % by SDS-PAGE/CBB staining
- Activity:
- The activity was measured by off-chip mobility shift assay. The enzyme was incubated with fluorecence-labeled substrate and Mg(or Mn)/ATP. The phosphorylated and unphosphorylated substrates were separated and detected by LabChip 3000. Substrate: Srctide. ATP: 1000 uM
- Storage Buffer:
- In 50 mM Tris-HCl, 150 mM NaCl, pH 7.5 (0.1% CHAPS, 1 mM DTT, 10% glycerol)
- Storage Instruction:
- Store at -80°C.
Aliquot to avoid repeated freezing and thawing.
- Quality Control Testing:
- Loading 1 ug protein in SDS-PAGE
- Note:
- Result of activity analysis
-
- Application Image
- Functional Study
- SDS-PAGE
- Entrez GeneID:
- 9113
- Protein Accession#:
- NP_004681.1
- Gene Name:
- LATS1
- Gene Alias:
- WARTS,wts
- Gene Description:
- LATS, large tumor suppressor, homolog 1 (Drosophila)
- Omim ID:
- 603473
- Gene Ontology:
- Hyperlink
- Gene Summary:
- The protein encoded by this gene is a putative serine/threonine kinase that localizes to the mitotic apparatus and complexes with cell cycle controller CDC2 kinase in early mitosis. The protein is phosphorylated in a cell-cycle dependent manner, with late prophase phosphorylation remaining through metaphase. The N-terminal region of the protein binds CDC2 to form a complex showing reduced H1 histone kinase activity, indicating a role as a negative regulator of CDC2/cyclin A. In addition, the C-terminal kinase domain binds to its own N-terminal region, suggesting potential negative regulation through interference with complex formation via intramolecular binding. Biochemical and genetic data suggest a role as a tumor suppressor. This is supported by studies in knockout mice showing development of soft-tissue sarcomas, ovarian stromal cell tumors and a high sensitivity to carcinogenic treatments. [provided by RefSeq
- Other Designations:
- LATS (large tumor suppressor, Drosophila) homolog 1,LATS homolog 1
- Related Disease